Fig. 5.

HETE concentrations in the culture medium in the presence of cells. Quantification of 5-, 8-, 11-, 12- and 15-HETE concentrations in basal, adipogenic and osteogenic medium, corresponding to cell cultures maintained in the absence of PUFA (controls) or treated with 20 μM of arachidonic acid (AA), 20 μM of AA plus 20 μM of DHA (AA + DHA) or 20 μM of AA plus 20 μM EPA (AA + EPA), at days 10 and 21 after differentiation start. *p < 0.05 in relation to the cultures maintained in basal medium for the same treatments and time; #p < 0.05 in relation to the cultures maintained in same medium and treatments at day 10