Table 1.
Primers for PCR amplification of each gene studied
Primers | Sense primer | Antisense primer |
---|---|---|
ACO | 5′-TC TCT GTG GTT GCT GTG GAG TCA-3′ | 5′-TC GGA TGC TTC CTT CTC CAA GGT-3′ |
CPT-1b | 5′-CAA ACA TCA CTG CCC AAG CTT-3′ | 5′-GGC CGC ACA GAA TCC AAG T-3′ |
PGC-1α | 5′-CA AAG CTG AAG CCC TCT TGC-3′ | 5′-GTT TAG TCT TCC TTT CCT CGT GTC C-3′ |
PPAR-α | 5′-GCT ACT TCG AGT CCC CTT GAG-3′ | 5′-CCA GCG TCG CTT CAG TTC C-3′ |
CD36 | 5′-GGT GTG CTC AAC AGC CTT ATC-3′ | 5′-TTA TGG CAA CCT TGC TTA TG-3′ |
TFAM | 5′-CAC GAG CCC TGG AGT ACC C-3′ | 5′-CCA CAT TCC CCG GAA CAG C-3′ |
COX-2 | 5′-AAC AAT TCT CCC AGC TGT CAT TC-3′ | 5′-AGT CAA AGC ATA GGT CTT CAT AGT C-3′ |
UCP3 | 5′-TAC ACC TCC TGA CCT TGG CTA G-3′ | 5′-CTG TTG TGG GAG GCA CTT CTG-3′ |
18S | 5′-GTG GGC CTG CGG CTT AAT-3′ | 5′-GCC AGA GTC TCG TTC GTT ATC-3′ |
ACO acyl-coenzyme A oxidase, CPT-1b carnitine palmitoyl transferase-1b, PGC-1α peroxisome proliferator-activated receptor gamma coactivator 1-α, PPAR-α peroxisome proliferator-activated receptor α; CD36 cluster of differentiation 36, TFAM transcription factor A; mitochondrial; COX-2 cyclooxygenase-2, UCP 3 uncoupling protein 3