Table 2.

Down-regulated genes by hgm-NDF-LC₅₀ and SCFA-LC₅₀ treatments compared with untreated HT-29 cells

Symbol	GenBank	Biological function	Fold regulation
			hgm-FNDF-LC50	SCFA-LC50
Regulation of apoptosis
BAX	NM_004324	Induction of apoptosis	−2.36	−1.57
BCL2	NM_000633	Anti-apoptosis	−1.25	−1.37
BCL2A1	NM_004049	Anti-apoptosis	−5.54	−1.44
BIRC5	NM_001168	Anti-apoptosis	−24.42	–
CRADD	NM_003805	Induction of apoptosis	−4.26	−2.12
ESR1	NM_000125	Induction of apoptosis, Inhibition of cell proliferation	−12.04	−11.82
FADD	NM_003824	Induction of apoptosis	−2.11	−1.54
LRDD	NM_018494	Induction of apoptosis	−2.23	−1.56
TP53AIP1	NM_022112	Induction of apoptosis	−4.82	−1.88
TP63	NM_003722	Induction of apoptosis	−1.02	−3.93
TRAF2	NM_021138	Anti-apoptosis	−2.85	−1.63
Regulation of cell cycle
BTG2	NM_006763	Negative regulation of cell cycle and proliferation	−2.14	−1.39
CCNB2	NM_004701	Cell cycle checkpoint	−1.49	−1.07
CCNH	NM_001239	Control of transcription and cell cycle	−1.69	−4.45
CDC2	NM_001786	Regulation of cell cycle	−3.16	−9.15
CDK4	NM_000075	Cell proliferation	−1.19	−1.07
CDKN2A	NM_000077	Cell cycle arrest	−3.81	–
CHEK1	NM_001274	Cell cycle checkpoint	−10.48	−1.04
E2F1	NM_005225	Regulation of cell cycle, apoptosis	−5.54	−5.40
E2F3	NM_001949	Regulation of cell cycle	−3.25	–
IFNB1	NM_002176	Negative regulation of cell proliferation	−3.18	−2.35
IGF1R	NM_000875	Regulation of cell cycle	−2.16	–
MYOD1	NM_002478	Negative regulation of cell cycle, differentiation	−2.50	−1.16
NF1	NM_000267	Negative regulation of cell cycle	–	−3.69
SESN2	NM_031459	Negative regulation of cell cycle	−3.23	−2.13
TADA3L	NM_006354	Induction of apoptosis, negative regulation of cell cycle	−2.68	−1.54
TP53	NM_000546	Induction of apoptosis, negative regulation of cell cycle	−84.45	−42.62
TP73	NM_005427	Induction of apoptosis, negative regulation of cell cycle	−10.27	−2.63
WT1	NM_000378	Negative regulation of the cell cycle	−6.63	−1.79
Cell proliferation
CDC25A	NM_001789	Control of cell proliferation	−14.12	−5.25
CDC25C	NM_001790	Control of cell proliferation	−6.32	–
EGR1	NM_001964	Other genes related to cell growth, proliferation and differentiation	–	−2.30
HK2	NM_000189	Cell proliferation	−2.79	−3.17
MDM2	NM_002392	Negative regulation of cell proliferation, induction of apoptosis	−24.42	−30.55
MYC	NM_002467	Cell proliferation	−5.54	−2.65
NFKB1	NM_003998	Cell proliferation, anti-apoptosis	−25.11	−12.41
PRC1	NM_003981	Cell proliferation	−4.63	−1.05
STAT1	NM_007315	Regulation of apoptosis and cell proliferation	−3.39	−2.61
Cell growth and DNA repair
BRCA1	NM_007294	Tumor suppressor gene, DNA repair	−20.11	−8.19
BRCA2	NM_000059	Tumor suppressor gene, DNA repair	−6.41	−5.25
DNMT1	NM_001379	Regulation of cytosine methylation	−17.39	−32.30
PPM1D	NM_003620	Cell growth and anti-apoptosis	–	−2.09
XRCC5	NM_021141	DNA repair genes	−2.23	−4.67

Results were normalized to housekeeping genes, and values represent the degree of changes in mRNA for treated HT-29 cells relative to untreated HT-29 cells. P = 0.05 compared with the control

– Not applicable