External anatomical abnormalities in GKirKO mice and puberty assessment. A, External anatomic abnormalities were not observed in GKirKO female mice. B, GKirKO males exhibited microphallus and decreased anogenital distance. Delayed puberty in GKirKO mice. C, Graphic representation of the time course of the day of VO in females (n = 5–12). D, Time course of the day of PPS in males (n = 7–15). E, Body weight change over time in female mice (n = 4–7). F, Body weight change over time in male mice (n = 9).