Figure 3. P. yoelii challenge of γ^−/−and CD32b^−/− mice.

Challenge outcome in BALB/c, γ^−/−, C57BL/6, and CD32b^−/− mice was investigated. All mice (n=6/group) were immunized i.m. with Ad-M PyMSP1₄₂. PyMSP1₁₉ ELISA titers were determined in (A) BALB/c and γ^−/− mice and (D) C57BL/6 and CD32b^−/− at 14, 55, and 70 days post adenovirus immunization (MVA boost occurred on Day 56). At Day 70, all mice were challenged i.v. with 10⁴ P. yoelii YM pRBCs. Blood-stage parasitemia is reported as the percentage of infected RBCs over time in (B) BALB/c and γ^−/− groups and (E) C57BL/6 and CD32b^−/− groups. AUC analysis for Days 3–5 (D3–5) was conducted on (C) parasitemia plots for BALB/c and γ^−/− mice and (F) plots from C57BL/6 and CD32b^−/− mice. Lines on dot plots represent median values. †Animal being culled after reaching the humane end-point of 50% blood-stage parasitemia.