Figure 5. ADRB and P. yoelii malaria challenge.

BALB/c or γ^−/− mice were immunized i.m. with Ad-M PyMSP1₄₂ and challenged 2 weeks later with 10⁶ P. yoelii 17XNL pRBCs. (A) Blood-stage parasitemias in BALB/c and γ^−/− groups are reported as the percentage of infected RBCs. (B) AUC analysis of parasitemia between Days 3 and 20 was conducted for naive/nonvaccinated (●) and immunized (○) groups. (C) Fifty-five days after challenge, ADRB activity of mouse serum against PyPEMS was assessed as before. (D) Postchallenge anti-PyPEMS ADRB activity was plotted against AUC, calculated between Days 3 and 20. Lines on dot plots represent medians, bar charts represent mean of two replicates for each sample, and line on scatter plot represents Spearman rank correlation. †Animal being culled after reaching the humane end-point of altered behavior due to treatment.