Figure 7. CD4⁺ and CD8⁺ cells are increased, but Tregs are reduced in STAT3-cKO mice compared with WT mice tumors.

The proportions of CD4⁺, CD8⁺, and CD25⁺Foxp3⁺ cells in Smo* WT versus STAT3-cKO tumors were determined by flow cytometry. For Tregs, cells were first gated on the CD4⁺ population. (A) Representative FACS plots and (B) the average proportions of these cells (n=8). Student's t-test, *P < 0.05; **P < 0.01.