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. 1972 Oct;69(10):2885–2889. doi: 10.1073/pnas.69.10.2885

Biochemically Differentiated Neoplastic Clone of Schwann Cells

S E Pfeiffer *, W Wechsler †
PMCID: PMC389667  PMID: 4342966

Abstract

Four clonal lines have been established in tissue culture from a transplantable tumor of cervical nerve-root induced transplacentally with ethylnitrosourea in an inbred BD-IX rat strain. One clone (RN-2) has several biochemical properties of the nervous system that led to its classification as a Schwann cell line, namely (a) the synthesis of the nervous system specific protein S-100, (b) a high specific activity of 2′,3′-cyclic nucleotide-3′-phosphohydrolase, an enzyme present in high specific activity only in glial cells, and (c) the presence of a basic protein related by immunological crossreaction, molecular size, and amino-acid composition to the encephalitogenic protein from beef brain myelin. Clone RN-2 has a high plating efficiency, a doubling time of about 20 hr, and a mean of 43 chromosomes of normal morphology. Clone RN-2 has also been inoculated subcutaneously into syngeneic rats and grown as a clonal tumor with good growth properties.

Keywords: S-100 protein; 2′,3′-cyclic nucleotide-3′-phosphohydrolase; myelin basic protein; peripheral nervous system

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Selected References

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