Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 15;7:27. doi: 10.1186/1756-3305-7-27

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Liu et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Western blotting analysis of rEnGAM22. The expressed protein was separated by SDS-PAGE and transferred to NC membranes, at the same time the wild type vector transferred bacterial was used as control. In (A) and (B), anti-6 × His tag monoclonal antibody (A) and mouse anti-GAM22 polyclonal antibody (B) were used as first antibody, individually. Protein marker (lane 1), the control (lane 2) and the recombinant protein (lane 3). (C) The convalescent sera from chickens were used as first antibody, anti-E. necatrix (lane 1), anti-E. tenella (lane 2) and anti-E. maxima (lane 3). (D) The gametocyte extracts were detected by the mouse anti-rEnGAM22 antibody. Gametocyte extracts (lane 1), protein marker (lane 2), and purified rEnGAM22 (lane 3).