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. 2014 Jan 7;5:2977. doi: 10.1038/ncomms3977

Figure 5. miR-181a is a novel modulator of the TGF-β signalling pathway in EOC.

Figure 5

(a) Global canonical pathway analysis. RNA-sequencing data sets from A2780 pBABE and p181a 1 cell lines were analysed using the Ingenuity Pathways Analysis software. Statistical significance is expressed as a P-value calculated using the right-tailed Fisher’s exact test. (b) Western blotting for the TGF-β receptor, activin receptor and Smad proteins assessing TGF-β pathway activity in miR-181a-overexpressing and control A2780 cells. (c) TGF-β transcriptional activity was assessed using both the firefly luciferase-based p3TP (left) and SBE4 (right) reporter constructs. Student’s t-test, ***P≤0.001 (n=3). Error bars, s.d. (d) SBE4 reporter activity (top) and phosphorylated Smad2 (P-Smad2) protein expression (bottom), as show by western blotting, in SKOV3 cells transduced with miR-181a decoy compared with the control decoy. Student’s t-test, ***P≤0.001 (n=3). Error bars, s.d. (e) Type-I TGF-β receptor (TGF-β RI) inhibitor, SB431542, effects on miR-181a-mediated activation of TGF-β signalling as measured by P-Smad2 protein expression and suppression of Smad7 protein expression as shown by western blotting.