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. 1972 Oct;69(10):2937–2941. doi: 10.1073/pnas.69.10.2937

Mechanism of Hormonal Induction of Tyrosine Aminotransferase Studied by Measurement of the Concentration of Growing Enzyme Molecules

Walter A Scott 1, Robert Shields 1, Gordon M Tomkins 1,*
PMCID: PMC389678  PMID: 4404146

Abstract

Proteins labeled in cultured hepatoma cells grown in the presence of radioactive amino acids and pactamycin consist primarily of growing polypeptide chains initiated before addition of the antibiotic. Nascent tyrosine aminotransferase (EC 2.6.1.5) molecules were labeled in this way, and their radioactivity after completion was measured immunologically. Cells exposed to the synthetic adrenal steroid, dexamethasone, contain 10- to 18- times as much nascent enzyme as uninduced cells. These results indicate that induction control is not at the level of polypeptide-chain elongation or release, but probably operates on either the amount of messenger RNA or the rate of specific polypeptide-chain initiation.

Keywords: pactamycin, hepatoma cells, dexamethasone

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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