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High (panel A) and low (panel B) microvessel area (MVA) by AQUA. We used a cocktail of anti-cytokeratin and anti-carbonic anhydrase-9 conjugated to Cy2 to create a tumor mask (green), and anti-CD-34 conjugated to Cy5 (red) to identify microvessels. An example of a patient with high MVA is shown in panel A, and an example of low MVA in panel B. The corresponding MVA scores were 28.37% and 3.31%.
