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. 2014 Jan 14;10:22. doi: 10.1186/1746-6148-10-22

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Copyright © 2014 Abera et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sensitivity of conventional RT-PCR based on TCID₅₀/ml by using NP3/NP4 primer pairs. Lane M-100 bp ladder; Lane-1, 10⁵ TCID 50/ml; Lane-2, 10⁴ TCID₅₀/ml; Lane- 3, 10³ TCID₅₀/ml; Lane-4, 10² TCID₅₀/ml; Lane-5, 10 TCID₅₀/ml; Lane-6, 1 TCID₅₀/ml; Lane-7, 0.1 TCID₅₀/ml. Product size, 351 bp.