Table 2.
Matrix effect and extraction recovery for the yakuchinone A and IS in rat plasma (n = 5)
|
Nominal conc. (ng/ml) |
Peak area (× 10
4
) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
Set 1 |
|
Set 2 |
|
Set 3 |
|
Matrix effect |
|
Extraction efficiency |
|
|
| Mean ± SD | RSD | Mean ± SD | RSD | Mean ± SD | RSD | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |
| Yakuchinone A | ||||||||||
| 4 |
7.45 ± 0.15 |
2.04% |
6.43 ± 0.04 |
0.70% |
5.18 ± 0.12 |
2.28% |
86.3 |
1.95 |
80.5 |
2.30 |
| 80 |
113 ± 6 |
5.05% |
111 ± 3 |
2.59% |
79.9 ± 1.2 |
1.56% |
98.5 |
6.95 |
71.7 |
2.89 |
| 800 |
930 ± 61 |
6.56% |
884 ± 19 |
2.11% |
789 ± 12 |
1.50% |
95.5 |
8.54 |
89.2 |
1.19 |
| IS | ||||||||||
| 80 | 80.7 ± 7.8 | 9.65% | 89.7 ± 1.9 | 2.16% | 52.7 ± 2.5 | 4.66% | 112 | 9.30 | 58.8 | 3.68 |