Table 1.
Comparison of statistics including read lengths, number of reads and number of bases for the raw 454 reads, with SMART adapters still attached, for Roche gsRunProcessor shotgun signal-processing pipeline versions 2.3 and 2.6. N50 values for versions 2.3 and 2.6, respectively, indicate that 50 % of the bases are in lengths of 407 and 398 bases or greater
| Roche signal pipeline version | 2.3 | 2.6 |
|---|---|---|
| Minimum read length | 40 | 40 |
| Maximum read length | 1,168 | 1,059 |
| Mean read length | 311.3 | 304.4 |
| Standard deviation of read length | 155.3 | 153.9 |
| Median read length | 324 | 315 |
| N50 read length | 407 | 398 |
| Number of reads | 385,856 | 494,391 |
| Number of reads in N50 | 123,116 | 156,749 |
| Number of bases in all reads | 120,135,078 | 150,473,196 |
| GC content of reads | 34.21 % | 34.02 % |