Table 1.
Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes
|
Primer sequence |
|||
|---|---|---|---|
| Forward | Reverse | ||
|
qAMP |
H19
|
5′-AGCCGTTGTGAGTGGAAAGA-3′ |
5′-CATAGCGGCTTCGGACATT-3′ |
|
Snrpn
|
5′-CTCCTCAGAACCAAGCGTCT-3′ |
5′-ATTCCGGTCAGAGGGACAGA-3′ |
|
|
Peg3
|
5′-GGTGTCCCGCAGCCCTTG-3′ |
5′-CGGAGCACAGCACTCTACGC-3′ |
|
| chr9:106724005-106724149 |
5′-GATCTATTCCTTCCTTTACTTT-3′ |
5′-TCCTGGGAAATGAAGTTT-3′ |
|
|
Peg3
|
qRT-PCR |
5′-TTGGACTGGACAGAGATGATGACA-3′ |
5′-ATTCTGGTATGACTCGGCATCCT-3′ |
|
Snrpn
|
5′-AGGCCCATCCCAGCAGGTCAT-3′ |
5′-GCGGGTACTGGGTTGGGGCTC-3′ |
|
|
H19
|
5′- CTTGTCGTAGAAGCCGTCTGTTC-3′ |
5′- GTAGCACCATTTCTTTCATCTTGAGG-3′ |
|
| Ppia | 5′- CGCGTCTCCTTCGAGCTGTTTG-3′ | 5′- TGTAAAGTCACCACCCTGGCACAT-3′ | |