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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Mol Recognit. 2014 Feb;27(2):82–91. doi: 10.1002/jmr.2336

Table 1.

Thermodynamic parameters for the binding of wildtype DB domain (DB_WT) of EGR1 to DNA at pH 7 and 25°C under various NaCl concentrations

[NaCl] / mM Kd / nM ΔH / kcal.mol−1 TΔS / kcal.mol−1 ΔG / kcal.mol−1
0 271 ± 35 −26.70 ± 0.61 −17.72 ± 0.69 −8.97 ± 0.08
100 314 ± 39 −19.01 ± 0.17 −10.13 ± 0.24 −8.88 ± 0.07
200 266 ± 66 −16.32 ± 0.16 −7.33 ± 0.31 −8.99 ± 0.15
300 421 ± 49 −14.27 ± 0.15 −5.57 ± 0.22 −8.71 ± 0.07
400 2441 ± 325 −14.20 ± 0.72 −6.54 ± 0.64 −7.67 ± 0.08
500 10119 ± 1228 −12.99 ± 0.37 −6.17 ± 0.44 −6.82 ± 0.07

The binding stoichiometries to the fits agreed to within ±10%. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation.