. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: J Mol Recognit. 2014 Feb;27(2):82–91. doi: 10.1002/jmr.2336

Table 2.

Thermodynamic parameters for the binding of wildtype DB domain (DB_WT) of EGR1 to DNA at pH 7 and 25°C under various KCl concentrations

[KCl] / mM	K_d / nM	ΔH / kcal.mol⁻¹	TΔS / kcal.mol⁻¹	ΔG / kcal.mol⁻¹
0	271 ± 35	−26.70 ± 0.61	−17.72 ± 0.69	−8.97 ± 0.08
100	345 ± 47	−17.31 ± 0.75	−8.48 ± 0.67	−8.83 ± 0.08
200	324 ± 50	−11.33 ± 0.25	−2.47 ± 0.15	−8.87 ± 0.09
300	758 ± 265	−6.78 ± 0.26	+1.60 ± 0.47	−8.37 ± 0.21
400	2070 ± 251	−5.95 ± 0.55	+1.81 ± 0.48	−7.76 ± 0.07
500	10534 ± 784	−4.45 ± 0.23	+2.35 ± 0.18	−6.80 ± 0.04

The binding stoichiometries to the fits agreed to within ±10%. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation.