Skip to main content
. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Nature. 2013 Jul 17;500(7462):345–349. doi: 10.1038/nature12303

Figure 4. Igf1r regulation and rescue by genetic blockage of Igf2-Igf1r signaling.

Figure 4

a, miR-675 analysis by qRT-PCR (n=3). b, Frequency of CD34− vs. CD34+ LSK cells 8W post-lentiviral infection. c, Immunoblot analysis of Igf1r in sorted BM cells (positive and negative for GFP). d–e, Single-cell immunostaining of Igf1r 8W post-lentiviral infection and its quantitation (n=4). f, Representative FACS plot with frequency (n=4) g, absolute number and h, cell-cycle analysis. i, Pan-FoxO staining (n=3). j, Donor engraftment 12W post transplantation. RNA sequencing analysis in sorted stem cells. k, imprinted genes, l–m, Wnt and BMP signaling n, heatmap of miRNA expression. Error bars s.e.m.