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. 2014 Jan 21;4:3791. doi: 10.1038/srep03791

Figure 6. DNA replication activity of gene edited cells.

Figure 6

Unsynchronized and synchronized cells were electroporated with either 13.5 ug 72NT or 2 ug TALEN plus 1.35 ug 72NT. Following a 48 hr recovery period, 10 uM of EdU was added to each sample and incubated for 18 hours (>one DNA replicative, division cell cycle). Immediately after EdU incubation, Click-iT EdU assay was performed to identify actively replicating cells by FACS. Quadrant 1 displays replicating but uncorrected cells; quadrant 2, replicating and corrected; quadrant 3 is nonreplicating and uncorrected; and quadrant 4 is nonreplicating and corrected. Percentages of cells in each quadrant were calculated automatically by FACSARIA II and are presented adjacent to each profile.