Table 1. Recovery, matrix effect, precision and accuracy for the determination of MGN in plasma by HPLC-MS/MS.
| Nominal concentration (μg/ml) | Recovery (%) | Measured concentration (μg/ml) | Accuracy (%) | Matrix effect (%) | Intra-day precision (RSD%) | Inter-day precision (RSD%) |
|---|---|---|---|---|---|---|
| 2.5 | 92.27 ± 0.66 | 2.603 ± 0.104 | 104.13 | 93.76 ± 5.57 | 4.32 | 7.53 |
| 1 | 91.60 ± 4.90 | 1.039 ± 0.040 | 103.90 | 96.57 ± 1.14 | 4.19 | 6.67 |
| 0.05 | 98.64 ± 6.66 | 0.049 ± 0.002 | 98.75 | 93.89 ± 9.06 | 3.82 | 5.18 |
Notes: Data are based on analysis of six replicates (n = 6) on three separate days.