Figure 2. Stromal compartment specific immune cell infiltration in human PDAC.

Proportion of immune cell infiltrate was determined as shown in Supplementary Figure 1-4. Juxtatumoral stromal (within 100μm of tumor cells (single or islands /ducts)) and panstromal (the rest of the tumor stroma) were defined. Each data point represents a single patient (median scores of all TMA cores (n=6)) and lines represent median for the cohort of patients. Comparison of immune cell infiltration in these two PDAC stromal compartments was made for CD4⁺ (A), CD8⁺ (B), FoxP3⁺ (C), CD20⁺ (D), CD68⁺ (E) and Myeloperoxidase⁺ (F) cells, and also for CD3⁺ and CD56⁺ cells (Supplementary Figure 5). Whilst PDAC tissues demonstrate a significantly higher density in the juxtatumoral stroma relative to the panstromal compartment for CD68⁺ cells, the reverse was true for CD8⁺, FoxP3⁺ and CD20⁺ cells with an equal density for CD4⁺ and Myeloperoxidase⁺ cells implying a differential immune cell infiltration defect. Mann Whitney U test; p-values are two-tailed.