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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Gastroenterology. 2013 Jul 25;145(5):1121–1132. doi: 10.1053/j.gastro.2013.07.025

Figure 7. T-cell migration is mediated by CXCL12.

Figure 7

CXCL12 was knocked down in PSC cell line (PS1: A) and primary PSCs (B) with two distinct targeting siRNAs and appropriate controls. CXCL12 secretion was measured in conditioned media. PSC treated with siRNA for CXCL12 demonstrated significant reduction of CXCL12 secretion (A,B) which was equivalent to quiescent PSC levels. Migration of normal human donor CD8+ T-cells to conditioned media of CXCL12 knockdown PSC (C: PS1 cell line and D: primary PSC) demonstrated significant reduction, which was equivalent to qPSC levels. Similar results were found with PDAC patient CD8+ T-cells (E). PDAC patient CD8+ T-cells demonstrated significant upregulation of CXCR4 as measured by Flow-cytometry (F).

*** p< 0.001; ** p= 0.001 to 0.01; * p= 0.01 to 0.05. Comparisons were conducted with ANOVA with comparisons between columns using Bonferroni’s Multiple Comparison Test.