Fig. 7.

A monoclonal antibody against phospholamban (2D12) that inhibits SERCA2a-PLB interaction, like PLB phosphorylation, organizes stochastic sparks into synchronized, rhythmic, high power, spontaneous local Ca²⁺ wavelets (LCR’s) in permeabilized VM. (A) Representative confocal line-scan images in control and after 2 min application of 2D12 (13.1μg/1ml). (B) Respective Fast Fourier Transforms (FFT) of the local Ca²⁺ oscillations recorded in A. (C, D) Average amplitude of Ca²⁺ signals of individual LCR’s and of LCR ensembles in control and in response to 2D12. **P < 0.01, n = 6 cells.