Figure 1.
B-1a B cells from normal pregnant animals but not from pregnant animals suffering pregnancy disturbances inhibit Th17 T cell differentiation. CD4+CD25− naïve T cells (2 × 105) were cultured with mitomycin-c treated CD19+CD5+ B-1a B cells (1 × 105) (1:2) from non-pregnant, BALB/c mated (normal pregnant) or DBA/2J mated (developing pregnancy disturbances) CBA/J females without (A) or with (B) the addition of a Th17 cytokine differentiation cocktail. Levels of IL17 were assayed in supernatants. Data are expressed as Box and Whisker plots showing median. *P < 0.05 as analyzed by the non-parametric Kruskal–Wallis test.