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. 2014 Jan 21;5:6. doi: 10.3389/fimmu.2014.00006

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Copyright © 2014 Muzzio, Soldati, Rolle, Zygmunt, Zenclussen and Jensen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B-1a B cells from pregnant animals developing normal pregnancies and animals suffering pregnancy disturbances display similar levels of MHCII expressing B-1a B cells. (A) Representative contour and density plots displaying the gating strategy used to analyze the percentages of MHCII expressing CD19⁺CD23⁻CD5⁺ B-1a B cells in the PerC of non-pregnant and pregnant mice. (B) Graph depicts percentages of MHCII expressing CD19⁺CD23⁻CD5⁺ PerC B-1a B cells from non-pregnant as well as BALB/c (normal pregnant) and DBA/2J (pregnancy disturbances) pregnant CBA/J females. Data are expressed as Box and Whisker plots showing median. Differences were analyzed by the non-parametric Kruskal–Wallis test.