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. 2014 Jan 21;5:6. doi: 10.3389/fimmu.2014.00006

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Copyright © 2014 Muzzio, Soldati, Rolle, Zygmunt, Zenclussen and Jensen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD86 expressing B-1a B cells are diminished in pregnant mice developing normal pregnancies but not in those suffering pregnancy disturbances. (A) Representative contour and density plots depicting gating strategy used to quantify percentages of CD86 expressing CD19⁺CD23⁻CD5⁺ PerC B-1a B cells of non-pregnant as well as BALB/c (normal pregnant) and DBA/2J (pregnancy disturbances) pregnant CBA/J females. Graph shows percentages of CD86 expressing CD19⁺CD23⁻CD5⁺ PerC B-1a B cells. (B) Representative contour and density plots depicting gating strategy used to quantify the percentages of CD80 expressing CD19⁺CD23⁻CD5⁺ PerC B-1a B cells of non-pregnant as well as BALB/c (normal pregnant) and DBA/2J (pregnancy disturbances) pregnant CBA/J females. Graph shows percentages of CD80 expressing CD19⁺CD23⁻CD5⁺ PerC B-1a B cells from non-pregnant as well as BALB/c or DBA/2J mated CBA/J females. Data are expressed as Box and Whisker plots showing median. *P < 0.05 as analyzed by the non-parametric Kruskal–Wallis test.