PCR products corresponding to the variable regions of the mIgA-type anti-α2Ct antibody. A; A schematic illustrating relevant domains of the mIgA variant. B; PCR products encompassing DNA sequences that encode the variable regions of the heavy α (VH) and the light κ (VL) chains of the mIgA-type anti-α2Ct antibody. Note: Some sets of the PCR primers did not create any products due to the lack of homology with the variable regions of the template. Symbols: Fv; Fragment variable regions, CDR; Complementarity determining regions, α and κ; constant regions of the heavy α and light κ chains, respectively, Fab; Fragment antigen-binding, Fc; Fragment crystallizable region, Vα and Vκ; PCR products corresponding to the Fv regions of the α and κ chains, respectively.