Figure 4.

Kinetics of the binding of the anti-α2Ct antibody variants to procollagen I. In each panel, the curves represent association and dissociation events during analyzed binding interactions between procollagen I and antibody variants present at concentrations ranging from 12.5 nM to 200 nM. For each assay, the association rate constants (k_on) and the dissociation rate constants (k_off) were obtained, and the equilibrium dissociation constants (K_D) values were calculated from a ratio of k_off/k_on.