Table I.
Sets of primers employed to generate PCR products spanning the sequence encoding the VH of the heavy α chain of the mouse IgA.
| a Sets of forward PCR primers targeting the 5′ end of the sequence encoding the VH of the mouse heavy μ chain (Ig-Primer Set EMD Millipore/Novagen) | b Reverse PCR primers targeting the 5′ end of the sequence encoding the constant region of the mouse heavy α chain a |
|---|---|
| MuIgH5′-A | α1_1 GGGAGTGTCAGTGGGTAGATGGTG* |
| MuIgH5′-B | α1_2 TTCCCACTCTTTCCCCAGGTCACA |
| MuIgH5′-C* | α1_3 AGAGGCGAGGGCAGGTGGAAAGTT |
| MuIgH5′-D | α1_4 CAGAGGCGAGGGCAGGTGGAAAGT |
| MuIgH5′-E | α1_5 TCACGGATCTCCTTCTGGGCACTG |
| MuIgH5′-F |
a
Detailed information on these primers can be found in the manual for the Ig-Primer Set (EMD Millipore/Novagen).
b
Primers were designed based on the DNA sequence of the mouse IgA heavy α chain published by Tucker et al. [8].
*
A primer set that resulted in a PCR product encompassing the VH region of the α chain of mouse IgA (see Fig. 1).