Fig. 6.

Effect of TLR9 on chronic stress-induced changes of Th1 and Th2 cytokines. TLR9 KO mice and WT mice aged 6 to 8 weeks were subjected to a 12-h physical restraint stress for 2 d. Splenic lymphocytes were treated with Con A (5 µg/ml). After 36 h incubation, the culture supernatants were harvested. Cytokine production in the supernatants, TNF (A), IL-4 (B), IL-6 (C), MCP-1 (D), and IL-2 (E) was assessed using cytokine-specific sandwich ELISA kits. Means and SEs were calculated from three to five mice per group. * p < 0.01 compared with indicated groups.