Table 3.
GFP-fused in vivo folding assessment of the final (β/α)8 fold-based libraries.[a]
| Species | Mode of GFP fluorescence[b] | % cells with GDPDwt-GFP fluorescence[c] |
|---|---|---|
| GDPDmut | 24.6 | 0.01 |
| Control library | 15.4 | 1.4 |
| Folding-enriched library | 19.8 | 5.4 |
| GDPDwt | 100 | 98.5 |
[a]
Constructs were transformed into E. coli BL21(DE3) Rosetta cells. Prior to analysis, data were gated to exclude cell populations that matched fluorescence and scatter profiles of cells transformed with empty vector control plasmid.
[b]
Values normalized to the mode of GFP fluorescence of GDPDwt-GFP.
[c]
Wild type cells were gated on the forward scatter versus GFP contour plot to include ~98% of all wild type cells.