Fig. 3.
Expression of different type II marker cells on different extracellular matrix proteins after 7 days. (A) SPC, (B) SPA and (C) SPB expression in iPSC-derived ATII on collagen I (ColI), collagen IV (ColIV), fibronection (Fib), Mix of Fib, ColI, ColIV, and human ECM compared to Matrigel, quantified by qRT-PCR. The gene expression in iPS derived-ATII cells on different ECM proteins were compared to iPS derived-ATII cells on Matrigel. Ct values from three independent experiments from the triplicate PCR reactions for a gene of interest (SPB, SPC, and SPA) were normalized against average GAPDH Ct values from the same cDNA sample. Fold change of GOI transcript levels between iPS derived-ATII on each ECM protein and iPS derived-ATII cells on Matrigel equals 2–ΔΔCt, where ΔCt = Ct(GOI) –Ct(GAPDH), and ΔΔCt = ΔCt(ECM) - ΔCt(Matrigel ). Collagen IV induced significantly higher levels of SPC, SPB and SPA expression compared to each ECM proteins individually. (Bar indicate ± SEM and n= 3 independent experiments *denotes statistically significant difference p-value < 0.05).