Fig. 4.
(A–M) Immunofluorescence analysis of type I cell (ATI) markers, T1α and Caveolin-1 in cells derived from iPSCs (C1 and C2 clones), at day 7 cultured in bioreactor and grown in flasks. (A–D) shows T1α and Caveolin-1 staining in iPSC-derived ATI cells from both C1 and C2 clones cultured in the rolling bioreactor; (E–H) Merge with DAPI. (I–M) Immunofluorescent staining of alveolar type I marker, (I, L) T1α and (J, M) Caveolin-1 in differentiated ATI cells from C1 and C2 iPSC exposed to WNT inhibitors. Immunofluorescent staining of (K) Caveolin-1 and (N) T1α in iPSC-ATII cells submerged in media. Scale bar, 63 µm.