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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biomaterials. 2013 Oct 19;35(2):699–710. doi: 10.1016/j.biomaterials.2013.10.018

Fig. 6.

Fig. 6

mRNA levels of (A) AQ5 (B) caveolin-1, (C) T1 a and (D) SPC in iPSC-derived ATI cells cultured in the rolling bioreactor system (air–liquid interface, or ALI system) compared to cells submerged in media and cells submerged in media with addition of inhibitors. qRT-PCR data from three independent experiments expressed as quantification of mRNA normalized to GAPDH and compared to hATI cells that were derived from fresh human lung. Fold change of GOI transcript levels between iPS derived-ATI and human type I cells equals 2−ΔΔCt, where ΔCt = Ct(GOI) –Ct(GAPDH), and ΔΔCt = ΔCt(AETII) - ΔCt(ATII) (Bar indicate ± SEM and n= 3 independent experiments for qRT-PCR and flow cytometry). ‘a’ marks statistically significant difference (p < 0.05) when comparing gene expression in cells cultured in ALI only vs. cells submerged in media. ‘b’ marks statistically significant difference (p < 0.05) in gene expression in cells exposed to IWR-1 vs. cells submerged in media.

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