Fig. 8.

(A–C) Comparison of type I phenotypes induction in four different conditions (submerged in media, in ALI system, in ALI plus ICG-001 and ALI plus IWR-I) in iPC-derived ATI using quantitative RT-PCR. Applying IWR-1 and ICG-001 and ALI to iPC-derived ATII induced type I phenotypes in these cells. Comparison of the impact of combination of both ALI system and WNT inhibitor on type I markers expression by real time qRT-PCR showed higher level of type I markers expression in ALI+IWR-1 compared to when they cultured in ALI system only. Comparison of gene expression revealed no statistically significant difference between ALI only, ALI+ICG-001 and ALI+IWR-1. Bars indicate ± SEM and n= 3 independent experiments for qRT-PCR, ELISA and flow cytometry.