Table 1.
List of antibodies used in staining, flow cytometry and western blot for various experiments.
| Primary antibodies | |||
|---|---|---|---|
| Antigen | Type | Provider (Cat#) (lot#) | Application |
| Nkx2.1 | Rabbit polyclonal | Abcam (Cat# ab76013, Lot# GR76790-2) | ICC, IHC, FC |
| proSPB | Rabbit polyclonal | Millipore (Cat# ab3430, Lot# NG1820771) | ICC, FC |
| proSPC | Rabbit monoclonal | Millipore (Cat# ab3786, Lot# 2117989) | ICC, IHC, FC |
| SPA | Rabbit polyclonal | Millipore (Cat# ab3420, Lot# NG1888873) | ICC |
| SPC | Rabbit polyclonal | Santa Cruz (Cat# sc-13979, Lot# L1710) | ICC, IHC, FC |
| T1α | Monoclonal | Abcam (Cat# ab10288, Lot# GR47830-3) | IHC |
| AQ5 | Rabbit polyclonal | Abcam(Cat# ab18530) | ICC |
| Caveolin-1 | Rabbit monoclonal | Millipore(Cat#MAB4381, Lot# LV1512392) | ICC |
| CD54-PE | Mouse monoclonal | BD Pharmingen (Cat# 560971, Lot# 10609) | FC |
| Detection antibodies | |||
| Type | Provider (Cat#) (lot#) | Application | |
| Alexa Fluor® 555 goat anti-rabbit IgG (H+L), highly cross-absorbed | Invitrogen (Cat# A21429, Lot# 1010124) | ICC, IHC | |
| Alexa Fluor® 488 goat anti-rabbit IgG (H+L), highly cross-adsorbed | Invitrogen (Cat# A11034, Lot# 1008720) | ICC, IHC | |
| Alexa Fluor® 555 goat anti-mouse IgG (H+L), highly cross-adsorbed | Invitrogen (Cat# A21424, Lot# 1214852) | ICC, IHC | |
| APC Mouse IgG2a, κ isotype control | BD Pharmingen (Cat# 555576, Lot# 33828) | Isotype control | |
| PE Mouse IgG1, κ isotype control | eBiosciences (Cat# 12-4714-82, Lot# E01672-1630) | Isotype control | |
| Mouse IgG2a, κ isotype control FTIC– | eBiosciences (Cat# 11-4724-81, Lot# E00590-1630) | Isotype control | |
| Isotype FITC goat anti mouse Ig | (Cat# 611233, Lot# 039557) | ||
| PE mouse IgG κ isotype control | (Cat# 555749, Lot# 38193) | ||
Abbreviations: IHC, immunohistochemistry; ICC, immunocytochemistry; FC, flow cytometry; PE, phycoerythrin, APC, allophycocyanin; FITC, fluorescein isothiocyanate; SPA, surfactant protein A; SPB, surfactant protein B; SPC, surfactant protein C; Nkx2.1, NK2 homeobox 1.