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. 2014 Jan 3;192(3):979–984. doi: 10.4049/jimmunol.1301685

FIGURE 1.

FIGURE 1.

Backcrossing of leptin-deficient mice with MRL/Mp-Faslpr mice. (A) MRL/Mp-Faslpr mice were crossed with C57BL/6J-ob/+ mice to produce offspring heterozygous for ob (mutant leptin gene). After 10 backcrosses with MRL/Mp-Faslpr mice, the mice were intercrossed, and progeny were analyzed for wild-type and mutant leptin genes by PCR amplification of tail DNA. Lanes 1 and 2 show DNA from wild-type (+/+) mice, and lanes 3 and 4 show DNA from leptin mutant (ob/ob) mice. Wild-type primers were used in lanes 1 and 3, and mutant type primers were used in lanes 2 and 4. (B) Serum leptin concentrations in MRL/Mp-Faslpr-ob/ob mice and control littermates, as measured by ELISA. Data are mean ± SEM (n = 10/group). (C) Body weights of 20-wk-old MRL/Mp-Faslpr-ob/ob mice and control littermates. Data are mean ± SEM (n = 10/group).