Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 20;204(2):203–213. doi: 10.1083/jcb.201304076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Dong et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Disruption of axoneme integrity and dynein arm defects in sperm of Pih1d3^−/− mice. (A) TEM of the midpiece (a–d) and principal piece (e–h) of sperm collected from the cauda epididymis of adult WT (a and e) or Pih1d32/2 (b–d and f–h) mice. Boxed areas are shown at higher magnification in the insets. Red arrows and arrowheads indicate ODAs and IDAs, respectively, in WT sperm or their loss in Pih1d3^−/− sperm. Red asterisks indicate loss of the 9+2 structural organization of microtubules in Pih1d3-deficient sperm. Bars, 200 nm. (B) Quantitation of ODAs, IDAs, and peripheral doublet microtubules in sperm of WT and Pih1d3^−/− mice. The numbers of ODAs and IDAs in TEM sections of 14 WT sperm and 19 Pih1d3^−/− sperm were counted, and the average number of each per sperm was calculated: 0.89 ODAs and 2.05 IDAs per mutant sperm, compared with 8.07 ODAs and 6.00 IDAs per WT sperm. Peripheral-doublet microtubules in 39 Pih1d3^−/− sperm were also counted, with 17 sperm showing nine intact doublets (closed diamonds), 10 sperm showing nine abnormal doublets (open diamonds), and 12 sperm showing a reduced number of doublets (filled triangles).