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. 2014 Jan 20;204(2):203–213. doi: 10.1083/jcb.201304076

Figure 3.

Figure 3.

Pih1d3 expression during sperm development. (A) WT (control) and Pih1d3flox/+ embryos at E8.0 were stained with X-gal. Pih1d3 is not expressed in mouse embryos at E8.0. Bars, 0.5 mm. (B) Investigation of Pih1d3 and E230019M04Rik expression in the indicated visceral organs from adult WT mouse and adult Pih1d3−/− testis (negative control) by RT-PCR. β-Actin serves as a loading control. (C) X-gal staining of the testis of adult Pih1d3flox/+ or WT (control) mice. The sections were counterstained with nuclear fast red. Roman numerals indicate stages of the cycle of the seminiferous epithelium. ps, pachytene spermatocyte; rs, round spermatids; es, elongated spermatids. The arrowheads indicate elongating spermatids. Bars, 50 µm. (D) In situ hybridization analysis of Pih1d3 mRNA in the testis of newborn, 5-wk-old, and adult WT mice with an antisense probe. Insets show staining with the corresponding sense probe as a control. All sections were counterstained with nuclear fast red. Stages of the cycle of the seminiferous epithelium are indicated with roman numerals. Bars, 50 µm.