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. 2014 Jan 20;204(2):203–213. doi: 10.1083/jcb.201304076

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© 2014 Dong et al.

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Figure 5. — Down-regulation of dynein subunits in the testis of Pih1d3^−/− mice. (A) Immunoblot analysis of visceral organs from adult WT mouse and the sperm and testis of adult WT and Pih1d3^−/− mice with antibodies to Pih1d3. The Pih1d3 protein (∼30 kD) is indicated by the arrowhead. (B) Immunofluorescence staining of sperm and the testis of adult WT and Pih1d3^−/− mice with antibodies to Dnaic2. The abundance of Dnaic2 (red fluorescence) was reduced in the sperm and testis of Pih1d3^−/− mice compared with those of WT mice (white arrows). Nuclei were stained with DAPI (blue fluorescence). Images were taken with a 40× objective lens (sperm) and 20× objective lens (testis). Bars, 50 µm. (C) Immunoblot analysis of dynein subunits and α-tubulin (loading control) in the sperm and testis of adult WT and Pih1d3^−/− mice.