Fig. 3. Mapping of siRNA effects into a molecular parameter space of protein function.

(A) Coculture assay used to constrain the parameter estimation problem. (B and C) Single-cell calcium traces of control cells and cells expressing siRNA targeting PMCA1 (B) or SERCA2 (C). (D and E) Single-cell parameter estimates from cells in which PMCA1 (D) or SERCA2 (E) was knocked down (red) or from control cells (blue). Center 80% values from each condition are shown.