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. Author manuscript; available in PMC: 2014 Jan 21.
Published in final edited form as: Nat Chem Biol. 2013 May 5;9(7):422–427. doi: 10.1038/nchembio.1251

Figure 4. Kinetic competence of radical intermediate.

Figure 4

(a) Representative CW-EPR spectra from the rapid freeze-quench time course (green trace, 180 s; red trace, 45 s; blue trace, 15 s). (b) Time-dependent formation and decay of the A2503 radical species (solid triangles) and time-dependent formation of m8A (open triangles). Both radical and m8A formation were fitted to an A→B→C kinetic model. The extracted rate constants are reported in the text. Error bars, where indicated, denote the standard deviation from two independent reactions. CW-EPR spectra were collected as described in the Materials and Methods.