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. Author manuscript; available in PMC: 2014 Jan 21.
Published in final edited form as: J Immunol. 2010 Sep 1;185(7):3809–3813. doi: 10.4049/jimmunol.1000971

Figure 1.

Figure 1

MHC Class II expression in the lung. (A) MHCII on pulmonary CD45− non-hematopoietic cells. (B) Immunostaining for CD31 (FITC) and MHC II (Texas Red) in lungs of B6 chimeras after reconstitution with B6II− hematopoietic cells. (C) CD45 expression on MHCII-positive cells in lungs and mediastinal lymph nodes. (D) Invariant chain expression by pulmonary non-hematopoietic cells (black line-antibody; shaded grey isotype). (E) DQ-ovalbumin processing and cleavage as identified by green fluorescence in live CD45−MHCII+ cells (thick green line) compared to cells fixed in 5% paraformaldehyde prior to incubation (dotted green line) or unlabeled cells (shaded grey plot). (F) Substitution of donor with recipient-derived hematopoietic APCs and CD4+ T cells in left lung grafts. (G) MHC class II expression on non-hematopoietic cells in transplanted B6 and B6II− lungs. Analysis is representative of at least four separate experiments.