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Apoptosis of Jurkat cells treated with the zerumbone-loaded nanostructured lipid carrier as determined by flow cytometry after staining with rTdT. (A1–D1) Untreated Jurkat cells at 12, 24, 48, and 72 hours. (A2–D2) Jurkat cells treated for 12, 24, 48, and 72 hours. Cells in the lower quadrant (R2 and R3) are nonapoptotic and cells in the upper quadrant (R4) are apoptotic.
Abbreviations: TUNEL, Tdt-mediated dUTP nick-end labeling; FITC, fluorescein isothiocyanate.
