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. 2014 Jan 16;9:527–538. doi: 10.2147/IJN.S54346

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© 2014 Rahman et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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(A) Western blots showing the effect of the zerumbone-loaded nanostructured lipid carrier on levels of cell cycle proteins regulating apoptosis in Jurkat cells after 24, 48, and 72 hours(h). B-actin was used as the loading control. (B) Western blotting analysis of the zerumbone-loaded nanostructured lipid carrier in Jurkat cells. The level of each protein was measured and normalized to β-actin. The values are shown as the mean ± standard deviation percentage of three independent experiments. Statistically significant differences (P<0.05) were found between treated cells and control cells in each group.

Abbreviations: Cyt-c, cytochrome c; Bcl-2, B cell lymphoma 2; Bax, Bcl-2 associated X protein; PARP, poly(adenosine diphosphate-ribose) polymerase; β-actin, Beta actin.