Table S3.
TUNEL flow cytometric analysis of Jurkat cells treated with ZER-NLC
| Cell condition | Cells (%)
|
|||||
|---|---|---|---|---|---|---|
| 24 hours
|
48 hours
|
72 hours
|
||||
| Control | ZER-NLC | Control | ZER-NLC | Control | ZER-NLC | |
| Viable | 96.63±0.4 | 77.75±0.09 | 97.63±0.21 | 68.97±0.7 | 95.68±0.24 | 60.31±1.07 |
| Apoptotic | 3.36±0.3 | 22.73±0.09* | 2.36±0.5 | 31.19±0.7* | 4.32±0.28 | 39.68±1.1* |
Notes: Values are expressed as the mean ± standard deviation of three different experiments. The data were analyzed using post hoc comparison test-one way analysis of variance, with means compared by the Tukey’s b test.
*
Significant (P<0.05) increase in apoptotic cells in ZER-NLC-treated group versus untreated control.
Abbreviations: TUNEL, Tdt-mediated dUTP nick-end labeling; ZER-NLC, zerumbone-loaded nanostructured lipid carrier.