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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Cell. 2013 Dec 5;155(7):1451–1463. doi: 10.1016/j.cell.2013.11.024

Figure 6. B. fragilis Treatment Corrects MIA-Mediated Changes in 4-ethylphenylsulfate (4EPS), a Microbe-Dependent Metabolite that Induces Anxiety-like Behavior.

Figure 6

(A) Relative quantification of metabolites detected by GC/LC-MS that are significantly altered by MIA and restored by B. fragilis treatment. n=8/group

(B) Serum concentrations of 4EPS detected by LC-MS. n=1, where each represents pooled sera from 3–5 mice

(C) Anxiety-like and locomotor behavior in the open field exploration assay. n=10/group

(D) Potentiated startle reflex in the pre-pulse inhibition (PPI) assay. n=10/group

S=saline+vehicle, P=poly(I:C)+vehicle, P+BF=poly(I:C)+B. fragilis, SPF=specific pathogen-free (conventionally-colonized), GF=germ-free, Veh.=vehicle (saline), 4EPS=4-ethylphenylsulfate. U.D.=undetectable. See also Figures S5, S6 and S7 and Tables S3 and S4.