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. 2014 Jan 21;9(1):e84853. doi: 10.1371/journal.pone.0084853

Figure 1. Genetic modification of the β-oxidation pathway in Saccharomyces cerevisiae.

Figure 1

The dashed line represents the original pathway; the solid line represents the modified pathway. The only acyl-CoA oxidase (encoded by the gene POX1) in the S. cerevisiae genome was deleted, and the POX2 gene from Yarrowia lipolytica, which encodes acyl-CoA oxidase with a preference for long chain acyl-CoAs, was expressed. To unblock the β-oxidation pathway, peroxisomal carnitine octanoyltransferase (CROT) from Mus musculus was also expressed to transport medium chain fatty acyl-CoAs out of peroxisomes.