Table 1. Representative fatty acid profiles of seed lipids from independent transgenic parental, GA7, mod-F and mod-G lines (T2 seeds with the highest DHA levels).
Parental | GA7 | Mod-F | Mod-G | |
16∶0 | 8.0±0.2 | 8.9±0.3 | 8.2±0.1 | 9.5±0.6 |
18∶0 | 3.5±0.2 | 4.1±1.1 | 3.5±0.0 | 4.0±0.1 |
18∶1ω7 | 1.4±0.1 | 1.7±0.1 | 1.2±0.1 | 1.2±0.1 |
20∶0 | 2.4±0.4 | 1.4±0.5 | 1.0±0.1 | 2.1±0.2 |
20∶1ω9/11 | 10.2±0.5 | 7.6±0.6 | 8.1±0.2 | 7.3±0.9 |
Minor | 5.6 | 3.1 | 2.3 | 4.3 |
OA | 9.8±0.2 | 6.3±0.2 | 9.5±0.2 | 4.4±0.3 |
LA | 18.1±0.6 | 6.7±0.1 | 8.4±0.3 | 11.5±1.3 |
ALA | 38.2±1.4 | 39.5±2.0 | 29.4±0.5 | 29.6±3.8 |
Omega-6 | ||||
GLA | – | – | 1.2±0.1 | 1.4±0.1 |
20∶2ω6 | 1.5±0.0 | 0.7±0.0 | 0.6±0.0 | 1.3±0.1 |
DGLA | – | – | – | – |
ARA | – | – | – | – |
22∶4ω6 | – | – | – | – |
DPA6 | – | – | – | – |
Omega-3 | ||||
SDA | – | 7.4±0.4 | 8.9±0.5 | 8.5±1.8 |
20∶3ω3 | 1.3±0.1 | 1.5±0.2 | 0.8±0.0 | 1.5±0.2 |
ETA | – | 0.3±0.1 | 0.4±0.1 | 0.4±0.2 |
EPA | – | 0.8±0.4 | 3.3±0.1 | 0.8±0.4 |
DPA | – | 0.4±0.0 | 0.8±0.1 | 0.7±0.4 |
DHA | – | 9.6±0.2 | 12.4±0.4 | 11.5±0.8 |
Δ12-des | 86% | 91% | 87% | 94% |
Δ15-des | 69% | 90% | 87% | 83% |
ω3 Δ6-des | – | 32% | 47% | 43% |
ω3 Δ6-elo | – | 60% | 66% | 61% |
ω3 Δ5-des | – | 97% | 98% | 97% |
ω3 Δ5-elo | – | 93% | 80% | 94% |
ω3 Δ4-des | – | 96% | 94% | 94% |
The errors denote standard deviation of triplicate samples. Apparent conversion efficiencies shown at the bottom describe the ω3 pathway and are calculated as the sum of product FAs/sum of substrate + product FAs.